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Compound Microscope

Microscope Used for Histology-Pkk

Microscope Anatomy

Parts of Microscope -PKK

Parts of Microscope

Manual of Microscope-Pkk

Microscope

Olympus Microscope-Pkk

PDF File

Histology All Slides 1- Low Power

Complete Histology Slides in Low Power for Quick Revision-PKK

PDF File

Histology All Slides 2- Low Power

Complete Histology Slides- 2, - in Low Power for Quick Revision-PKK

The Cell

Animal Cell -PKK

Cell Organelles

Different organelles of cell-PKK

Structure of Animal Cell

Animal Cell- Organelles-PKK

Different Cell-Histology

Squamous, Cuboidal and Columnar Cell -Pkk

Animal and Plant Cell

Compare Animal and Plant Cells-PKK

Eukaryotic Cell

Cell Membrane-PKK

Squamous Cell

Flattened Cell with Oval Basal Nucleus on basement Membrane-PKK

Cuboidal Cell

Cuboid shaped Cell with Central Round Nucleus-PKK

Columnar Cell

Tall Cells with Tall Oval Basal Nucleus which covers three fourth of length of cell-PKK

All Cell Histology

All Types of Cell -PKK

Columnar Cell High Power

Detailed Structure of Columnar Cell -PKK

Cuboidal Cell

Cuboid shaped Cell with Central Round Nucleus-PKK

Squamous Cell

Flattened Cell with Oval Basal Nucleus on basement Membrane-PKK

Cuboidal Cell

3 D view of Cuboidal cell-PKK

Cuboidal Cell -Thyroid

Cuboid shaped Cell with Central Round Nucleus-PKK

Squamous Cell- Glomerulus

Glomerulus of Kidney -Flattened Cell with Oval Basal Nucleus on basement Membrane-PKK

Cuboidal Cell - High Power

High Power- Cuboid shaped Cell with Central Round Nucleus-PKK

Epithelium Classification

Different Types of Epithelium-Pkk

Connective Tissue- Classification

Classification.-Connective tissue proper (loose and dense), supporting connective tissue (like cartilage and bone), and fluid connective tissue (like blood and lymph).

Connective Tissue- Classification2

Connective Tissue Proper Loose Connective Tissue: Fibers and cells are loosely arranged in a semi-fluid ground substance. Areolar: Binds organs together and is found beneath the skin. Adipose: Stores fat in specialized cells called adipocytes. Reticular: Forms a network of fibers in lymphatic tissue

Types of Connective Tissue

Loose, adipose , Dense Regular, Dense Irregular

C.T. Types

Loose, Dense, Regular, Blood

Different Connective Tissue

Fibroblasts, adipocytes, and macrophages), fibers (such as collagen, reticular, and elastic), and an amorphous ground substance

Skin- Connective Tissue

The main connective tissue in the skin is the dermis, which is a layer of dense, irregular connective tissue beneath the epidermis. It is composed primarily of collagen and elastic fibers, which provide strength and flexibility to the skin. The dermis also contains various cells, such as fibroblasts, and other structures like blood vessels, nerve endings, hair follicles, and glands.

Loose C.T.

Include fibroblasts and various immune cells like macrophages and mast cells, while the extracellular matrix is a mix of collagen, elastic, and reticular fibers.

Cells and Fibers- C.T.

Elastic fibres are made up of elastin and microfibrils which are made up of a mixture of elastin and glycoproteins, including fibrillin

Fibers of Connective Tissue

Elastic and reticular Fibers

Cartilage- C.T.

Composed of specialized cells called chondrocytes, which produce a gel-like matrix containing collagen fibers, proteoglycans, and other proteins

Loose C.T.

Areolar tissue - Characterized by a semi-fluid matrix with sparse collagen and elastic fibers, and is found beneath epithelia, surrounding blood vessels, and between muscles-PKK

Collagen and Reticular Fibers- C.T.

Fibroblasts are cells that produce collagen, and the most abundant type, Type I, is found in scar tissue, tendons, ligaments, and bones. Reticular connective tissue is a loose connective tissue that forms a supportive framework for organs, particularly those in the immune system, like the lymph nodes, spleen, and bone marrow.

Brown Adipose Tissue- C.T.

(BAT), is a specialized type of fat that generates heat to maintain body temperature, primarily through a process called non-shivering thermogenesis. It is found in newborns and adults, although in smaller amounts in adults, and its main function is thermogenesis,

Dense Regular Connective Tissue

A type of connective tissue where collagen fibers are arranged in parallel bundles, giving it high tensile strength in one direction. It is found in tendons, which connect muscle to bone, and ligaments, which connect bone to bone. This parallel arrangement allows the tissue to withstand strong pulling forces along its length.

1. Classification of Muscle

3 types—skeletal, cardiac, and smooth—based on the presence of striations, voluntary control, and cellular structure (nuclei, shape, and branching). Skeletal muscle is striated and voluntary; cardiac is striated and involuntary; smooth is non-striated and involuntary

2. Types of Muscle

Muscle tissue histology reveals three distinct types—skeletal, cardiac, and smooth

4. Skeletal Muscle- T.S.

Skeletal Muscle (Striated, Voluntary)Cell Shape: Long, cylindrical, unbranched fibers. Nuclei: Multinucleated; nuclei are located peripherally, just under the sarcolemma. Striations: Visible light (I-band) and dark (A-band) bands due to organized sarcomeres (myofibrils). Connective Tissue: Organized into epimysium (whole muscle), perimysium (bundles/fascicles), and endomysium (individual fiber

5. Skeletal Muscle-TS

Each muscle is a bundle of muscle fibres, each of which is a long multinucleated cell. Single mononucleated cells called myoblasts fuse together to form this multinucleated fibre (myotube) during development. This process also occurs to regenerate muscle fibres. The stripes come from the repeating sarcomeres.

6. T.S. Of Skeletal Muscle

The dark purple nuclei can be seen on the edges of the muscle fibres. Sarcolemma - cellular membrane Terminal cisterna - extension of sarcolemma that stores calcium T-tubules - invaginations of sarcolemma that transfer action potentials to the inside of the muscle cell Sarcoplasm - cytoplasm Sarcomplasmic reticulum - modified endoplasmic reticulum Actin, myosin - contractile elements Sarcomere - functional unit (made of actin and myosin) Accessory proteins - titin, tropomodulin, alpha-actinin, desmin, nebulin, dystrophin, myomesin

7. Skeletal Muscle Spindle

Tissue Sheaths --Endomysium - around single muscle fiber Perimysium - around multiple muscle fibers -> arranges them in fascicles Epimysium - around entire muscle

8. T.S Skeletal Muscle

There are three types of connective tissue sheaths named for their location. Endomysium surrounds individual muscle fibers. It is made up of a delicate layer of reticular fibers and permits only small-diameter nerve fibers and capillaries, thus acting as a site of metabolic exchange.

9. L.S. Skeletal Muscle

Perimysium is a slightly thicker layer of connective tissue consisting mainly of type I and III collagen and surrounds a group of fibers. This fiber group is referred to as a fascicle or bundle. Fascicles are the functional units of skeletal muscle tissue. The perimysium contains slightly larger blood vessels and nerve fibers than those traveling through endomysium

10. Skeletal Muscle-TS

Epimysium surrounds the entire collection of fascicles making up an individual muscle. This dense connective tissue made up of mainly type I collagen contains the neurovascular supply to the muscle

11. T.S Skeletal Muscle

The sarcomere is the functional unit of a skeletal muscle cell. Each sarcomere is about 2.5 micrometers in length. It is made up of multiple myosin and actin filaments oriented in parallel. The actin and myosin filaments overlap in certain places creating several bands and zones. A Z disc forms the boundary of the sarcomere on either side. Thin actin filaments project in either direction off of a Z disc but do not cross the entire length of the sarcomere. They are almost 8 nm in diameter and have tightly bound regulatory proteins called troponin and tropomyosin.

12. Smooth Muscle

In transverse section (TS) (cross-section), smooth muscle appears as a packed, mosaic-like arrangement of circular or oval cells of varying diameters. Only cells cut through the central nucleus show a round, dense nucleus surrounded by cytoplasm; others appear as simple cytoplasmic rings without a nucleus.

13. L.S. Smooth Muscle

Spindle-shaped, non-striated cells (3–10 µm thick, 20–200 µm long) with a single, central, cigar-shaped nucleus. In longitudinal sections, these fibers are aligned in parallel, showing a smooth, unbranched, and tapered structure that acts as involuntary, slow-contracting, and fatigue-resistant muscle.

14. Smooth Muscle Histology

Cell Shape: Fusiform (spindle-shaped) with tapering ends. Nucleus: Single, centrally located, oval to cigar-shaped nucleus. Striations: Absent (unlike skeletal muscle), giving the muscle a "smooth" appearance.

15. Histology of Smooth Muscle

Arrangement: Cells are packed tightly, connected by gap junctions that allow for synchronous contraction. Cytoplasm: Homogeneously eosinophilic (pink when stained with H&E) due to random, rather than arranged, contractile proteins (actin and myosin).

16. Smooth Muscle - L.S.

LOCATIONS-- Visceral (Single-unit) Smooth Muscle: Found in the walls of hollow organs (e.g., stomach, intestines, uterus, bladder) and blood vessels. Multi-unit Smooth Muscle: Found in areas requiring fine control, such as the iris of the eye and walls of large arteries

17. Smooth Muscle Histology

Function: These muscles are innervated by the autonomic nervous system to produce involuntary movements, such as peristalsis in the digestive tract and regulation of blood pressure in blood vessels

18. L.S. of Smooth Muscle

The smooth muscle fibers group in branching bundles. As opposed to skeletal muscle fibers these bundles do not run strictly parallel and ordered but consist in a complex system. Cell Shape: Fusiform (spindle-shaped) with tapering ends. Nucleus: Single, centrally located, oval to cigar-shaped nucleus. Striations: Absent (unlike skeletal muscle), giving the muscle a "smooth" appearance.